Journal: Obesity (Silver Spring, Md.)

Article Title: Adipose tissue CTGF expression is associated with adiposity and insulin resistance in people

doi: 10.1002/oby.22463

Figure Lengend Snippet: (A) Gene expression of connective tissue growth factor (CTGF) and adiponectin (ADIPOQ) was determined in human subcutaneous preadipocytes before (D0) and 8 days (D8) after induction of adipogenesis (n=4). (B) Microarray dataset obtained from the Gene Expression Omnibus (GEO) database (GSE8995) were used to evaluate CTGF and ADIPOQ expression in stromal vascular fraction (SVF) cells and adipocytes isolated from human subcutaneous adipose tissue samples (n=6). Data were log-transformed for the statistical analysis and back-transformed for presentation. Data are means ± SEM. *Value significantly different from D0 or SVF value, P<0.001.

Article Snippet: Human preadipocytes were treated with differentiation medium (#PT-8002; Lonza), and differentiated to mature adipocytes as described previously ( 29 ).

Techniques: Expressing, Microarray, Isolation, Transformation Assay

Journal: Nature metabolism

Article Title: Reverse gene-environment interaction approach to identify variants influencing body-mass index in humans

doi: 10.1038/s42255-019-0071-6

Figure Lengend Snippet: ( a ) Bright-field images of preadipocytes (top) and in vitro differentiated adipocytes (bottom) for unstained cells (left) and cells stained with oil red o (right). Images are representative examples from 2 independent experiments. ( b ) Heatmaps show log 2 fold-change (log 2 FC) of bins per million mapped reads (BPM) for preadipocytes compared to adipocytes in the three indicated peak sets. FDR was calculated (adjusting for n=154,647 ATAC-seq peaks) from the p -values of the QL F-test (see ) for differential accessibility between preadipocytes and adipocytes using ATAC-seq libraries from n=3 replicates per cell type. The top enriched transcription factor motif for the indicated peak set is listed (right). Enrichment p -values are derived from the hypergeometric enrichment test of the proportion of the given top de novo identified TF motifs in the 3 indicated peak sets compared with the background set (see ). ( c,d ) Read coverage (BPM) in one representative (out of n=3 replicates per cell type) preadipocyte (blue) and adipocyte (red) ATAC-seq library at the adipocyte-accessible ATAC-peaks in the promoters of ( c ) the adipocyte hormone gene, adiponectin ( ADIPOQ ) and ( d ) the adipocyte-specific marker, peroxisome proliferator-activated receptor gamma coactivator 1-alpha ( PPARGC1A ). PAd indicates preadipocytes; and Ad, adipocytes.

Article Snippet: We obtained and cultured the primary human white preadipocyte cells as recommended by PromoCell (PromoCell C-12731, lot 395Z024) for preadipocyte growth and differentiation into adipocytes.

Techniques: In Vitro, Staining, Derivative Assay, Marker